大戟中的肉豆蔻烷型二萜对黑色素瘤癌细胞具有细胞生长抑制活性和凋亡作用
Fitoterapia
(
IF
2.6
)
Pub Date : 2022-01-31
, DOI:
10.1016/j.fitote.2022.105138
Zeinab Yazdiniapour
1
,
Mina Mirian
2
,
Behzad Zolfaghari
3
,
Pegah Mehdifar
3
,
Mustafa Ghanadian
3
,
Virginia Lanzotti
4
Affiliation
Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran; Department of Pharmacognosy, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Pharmaceutical Biotechnology, Isfahan University of Medical Sciences, Isfahan, Iran.
Department of Pharmacognosy, Isfahan University of Medical Sciences, Isfahan, Iran.
Dipartimento di Agraria, Università di Napoli Federico II, Portici, Napoli, Italy.
Euphorbia gedrosiaca Rech.f.、Aellen & Esfand的植物化学分析。, 伊朗地方性大戟, 提供了四种 myrsinane 类型二萜聚酯的分离。两种新化合物 ( 1-2 ) 是基于肉豆蔻烷骨架，而其他 ( 3-4 ) 是已知的基于环肉豆蔻烷骨架的二萜。它们的化学结构通过光谱方法阐明，包括 1D 和 2D NMR 和 HRESIMS。对分离的化合物进行了测试，以评估它们对黑色素瘤细胞系 B16F10 和 A375 的细胞生长抑制活性和凋亡作用。化合物1–4的 IC 50值在 B16F10 上分别为 58.45、55.43、86.52 和 82.27 μM，在 A375 细胞上分别为 20.66、21.88、36.21 和 39.87 μM。未处理的细胞用作阴性对照（100% 细胞生长），5 nM 紫杉醇被视为阳性对照。
"点击查看英文标题和摘要"
Myrsinane-type diterpenes from Euphorbia gedrosiaca with cell growth inhibitory activity and apoptotic effects on melanoma cancer cells
Phytochemical analysis of Euphorbia gedrosiaca Rech.f., Aellen & Esfand., an Iranian endemic spurge, afforded the isolation of four myrsinane types diterpene polyesters. Two new compounds (1–2) were based on a myrsinane skeleton while the others (3–4) were known diterpenes based on a cyclomyrsinane backbone. Their chemical structures were elucidated by spectroscopic methods, including 1D and 2D NMR and HRESIMS. The isolated compounds were tested to evaluate their cell growth inhibitory activity and apoptotic effects on melanoma cell lines, B16F10 and A375. The IC50 values for compounds 1–4 were 58.45, 55.43, 86.52 and 82.27 μM, respectively, on B16F10, and 20.66, 21.88, 36.21 and 39.87 μM, respectively, on A375 cells. Non-treated cells were used as negative control (100% cell growth) and 5 nM Taxol were considered as a positive control.
更新日期：2022-02-03